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[Antipertussis immunity studied by immunoenzyme analysis].

T S Selezneva, E A Baeva, N V Tsvetkova

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |July 1, 1986
    PubMed
    Summary

    Antibody levels to Bordetella pertussis were measured in children receiving DPT vaccine. Immunized children showed higher antibodies to the whole bacterium and its protein, compared to its lipopolysaccharide component.

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    [The kit of reagents for polymerase chain reaction diagnostic of infections caused by B. pertussis, B. parapertussis and B. bronchiseptica].

    Klinicheskaia laboratornaia diagnostika·2013

    Area of Science:

    • Immunology
    • Vaccinology

    Background:

    • Pertussis remains a significant public health concern, necessitating effective vaccination strategies.
    • The Diphtheria, Tetanus, and Pertussis (DTP) vaccine is crucial for preventing pertussis.

    Purpose of the Study:

    • To quantify antibody levels against Bordetella pertussis and its fractions after DTP vaccination.
    • To compare immune responses to different vaccine batches and bacterial components.

    Main Methods:

    • Enzyme Immunoassay (EIA) was employed to determine antibody titers.
    • Antibodies were measured against whole, disintegrated B. pertussis, and its protein and polysaccharide fractions.
    • Control groups established baseline antibody levels.

    Main Results:

    • Immunized children exhibited significantly higher antibody levels to whole B. pertussis compared to controls.
    • Antibody responses were notably higher against the protein fraction than the lipopolysaccharide fraction of B. pertussis.
    • Variations in antibody levels were observed across different DTP vaccine batches.

    Conclusions:

    • DTP vaccination effectively elicits antibodies against Bordetella pertussis.
    • The immune response is predominantly directed towards the protein components of B. pertussis.
    • Further research may optimize vaccine composition for enhanced pertussis protection.

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