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Updated: Feb 25, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Virtual protein purification: A simple exercise to introduce ph as a parameter that effects ion exchange
Daniel D Clark1, Daniel J Edwards1
1Department of Chemistry and Biochemistry, California State University-Chico, Chico, California, 95929-0210.
None:
This article describes a simple exercise using a free, easy-to-use, established online program. The exercise helps to reinforce protein purification concepts and introduces undergraduates to pH as a parameter that affects anion-exchange chromatography. The exercise was tested with biochemistry majors at California State University-Chico. Given the versatility of the program, this work is also a model for instructors that wish to develop their own exercise to help teach other protein purification techniques. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(1):91-97, 2018.
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