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Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
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Engineering cell aggregates through incorporated polymeric microparticles.

Caroline C Ahrens1, Ziye Dong1, Wei Li1

  • 1Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, United States.

Acta Biomaterialia
|August 8, 2017
PubMed
Summary
This summary is machine-generated.

Microparticles (MPs) enhance cell aggregate function by controlling nutrient transport and signaling. This review details MP production and integration strategies for advanced tissue engineering and drug testing applications.

Keywords:
Cell aggregatesEx vivoExtracellular matrix (ECM)In vitro modelsMicroenvironmentMicroparticle

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cellular Biology

Background:

  • Ex vivo cell aggregates face nutrient transport and signaling limitations compared to native tissues.
  • Engineered environments often fail to replicate native tissue architecture and cues.
  • Microparticles (MPs) offer a solution for controlled presentation of soluble and tethered factors.

Purpose of the Study:

  • To review strategies for microparticle (MP) production and functionalization for cell aggregate co-culture.
  • To highlight techniques for integrating cells and MPs to control aggregate architecture and stability.
  • To discuss applications of MP-enhanced cell aggregates in tissue engineering and drug testing.

Main Methods:

  • Review of established and developing MP production strategies (materials, fabrication, functionalization).
  • Analysis of cell-MP integration techniques for targeted heterogeneity/homogeneity.
  • Examination of methods to engineer cell-particle and particle-particle interactions.

Main Results:

  • MPs can maintain soluble gradients and present tethered cues, supporting tissue development (cartilage, islets, nerves, vasculature).
  • MPs integrated with stem cells can direct in vivo expansion and differentiation.
  • MP co-cultures offer potential for in vitro tissue systems and drug testing platforms.

Conclusions:

  • Microparticle technology provides a versatile toolbox for developing advanced cell aggregates.
  • Careful consideration of material properties and biological context is crucial for successful MP-based co-cultures.
  • Future development will focus on enhancing MP capabilities for complex tissue engineering and personalized medicine.