Related Experiment Video
Updated: Feb 25, 2026

06:33
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Published on: October 29, 2019
10.8K
Nonlinear microscopy using cylindrical vector beams: Applications to three-dimensional imaging of nanostructures
Optics Express
|August 9, 2017
Summary
Cylindrical vector beams enable novel 3D imaging of nano-objects. This nonlinear microscopy technique advances the study of individual nanostructures in three dimensions.
Area of Science:
- Optics and Photonics
- Nanotechnology
- Microscopy
Background:
- Three-dimensional (3D) optical fields from cylindrical vector beams (CVBs) offer enhanced contrast for nano-object imaging.
- Previous applications were limited to 2D scanning or point-like objects.
Purpose of the Study:
- To demonstrate the first application of CVBs for 3D imaging of 3D nano-objects.
- To utilize the unique optical fields generated by CVBs for nanoscale structural analysis.
Main Methods:
- Employed CVBs with radial and azimuthal polarizations for focusing.
- Acquired 3D image scans of the second-harmonic generation (SHG) signal.
- Studied vertically-aligned semiconductor nanowires, leveraging their longitudinal electric field response.
Main Results:
- Successfully achieved 3D imaging of 3D nano-objects using CVBs.
- Demonstrated the capability to resolve nanostructures in three dimensions.
- Showcased the sensitivity of the SHG signal to the longitudinal electric field of nanowires.
Conclusions:
- CVBs provide a powerful new tool for 3D imaging of nanostructures.
- This technique combines nonlinear microscopy with CVBs for advanced nano-object characterization.
- Offers a novel approach for studying individual nano-objects in three dimensions.
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
921
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
921
Confocal Fluorescence Microscopy
21.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.4K
Two-Dimensional Microscopy in Microbiology
1.7K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.7K
Super-resolution Fluorescence Microscopy
14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Overview of Electron Microscopy
15.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
15.7K
Overview of Microscopy Techniques
17.2K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.2K

