Two novel cross‑protective antigens for bovine Pasteurella multocida

Area of Science:

• Veterinary Immunology
• Bacteriology
• Vaccine Development

Background:

• Pasteurella multocida causes significant economic losses in cattle production due to various diseases.
• Developing effective vaccines against P. multocida is crucial for protecting cattle health and the agricultural economy.

Purpose of the Study:

• To identify cross-protective outer membrane proteins (OMPs) of Pasteurella multocida serotypes A, B, and F for vaccine development.
• To screen and validate candidate antigens using immunoproteomics and bioinformatics.

Main Methods:

• Immunoproteomics approach to analyze OMPs from P. multocida serotypes A, B, and F.
• Linear trap quadrupole mass spectrometry and bioinformatics for candidate antigen screening.
• Cloning, expression, ELISA, and immunoblotting to analyze antigen reactogenicity.
• Infective animal models to determine vaccine efficacy and cross-protective potential.

Main Results:

• Identified three candidate proteins: rPmCQ2_2g0128, rPmCQ2_1g0327, and rPmCQ2_1g0020.
• rPmCQ2_2g0128 showed protective rates of 40%, 30%, and 20% against serotypes A, B, and F, respectively.
• rPmCQ2_1g0327 demonstrated protective rates of 50%, 40%, and 0% against serotypes A, B, and F, respectively.
• rPmCQ2_1g0020 provided 0%, 40%, and 30% protection against serotypes A, B, and F, respectively.

Conclusions:

• The proteins rPmCQ2_2g0128 and rPmCQ2_1g0327 show potential as vaccine candidates against bovine P. multocida serotypes A and B.
• This study is the first to identify cross-protective antigens by extracting OMPs from bovine P. multocida.