Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Second-Order Circuits01:17

Second-Order Circuits

3.7K
Integrating two fundamental energy storage elements in electrical circuits results in second-order circuits, encompassing RLC circuits and circuits with dual capacitors or inductors (RC and RL circuits). Second-order circuits are identified by second-order differential equations that link input and output signals.
Input signals typically originate from voltage or current sources, with the output often representing voltage across the capacitor and/or current through the inductor. For example, in...
3.7K
First-Order Circuits01:15

First-Order Circuits

4.0K
First-order electrical circuits, which comprise resistors and a single energy storage element - either a capacitor or an inductor, are fundamental to many electronic systems. These circuits are governed by a first-order differential equation that describes the relationship between input and output signals.
One common example of a first-order circuit is the RC (resistor-capacitor) circuit. These circuits are used in relaxation oscillators such as neon lamp oscillator circuits. When voltage is...
4.0K
Design Example: Capacitance Multiplier Circuit01:20

Design Example: Capacitance Multiplier Circuit

1.6K
In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.
1.6K
Network Function of a Circuit01:25

Network Function of a Circuit

946
Frequency response analysis in electrical circuits provides vital insights into a circuit's behavior as the frequency of the input signal changes. The transfer function, a mathematical tool, is instrumental in understanding this behavior. It defines the relationship between phasor output and input and comes in four types: voltage gain, current gain, transfer impedance, and transfer admittance. The critical components of the transfer function are the poles and zeros.
946
Neural Circuits01:25

Neural Circuits

3.0K
Neural circuits and neuronal pools are two of the main structures found in the nervous system. Neural circuits are networks of neurons that work together to carry out a specific task or process. They consist of interconnected neurons and glial cells, which provide structural and metabolic support.
Neuronal pools are collections of nerve cells with similar functions and interact through chemical and electrical signals. These pools include both interneurons (the central neural circuit nodes that...
3.0K
Circuit Terminology01:14

Circuit Terminology

3.0K
An electrical network is a system composed of interconnected elements, such as resistors, capacitors, inductors, and voltage or current sources. Unlike a circuit, an electrical network does not necessarily form a closed path. In other words, while all circuits can be considered networks due to their interconnected nature, not every network qualifies as a circuit.
A circuit, on the other hand, is also an interconnected system of electrical elements but must contain one or more closed paths.
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering drug-responsive replication machinery for precise control of self-amplifying RNA.

Nature biomedical engineering·2026
Same author

Advances in oncolytic viruses immunotherapy of hepatocellular carcinoma.

Discover oncology·2026
Same author

Connecting transcriptional control to RNA velocity and cell fate.

Cell·2026
Same author

Current Progress and Future Outlook for Synthetic Gene Circuits in Cardiovascular Therapy.

Biomolecules·2026
Same author

Tracking-seq: a universal off-target detection approach for CRISPR-Cas genome editing.

Nature protocols·2026
Same author

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models.

Advanced healthcare materials·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

13.0K

A Modular Approach to Building Complex Synthetic Circuits.

Yinqing Li1, Ron Weiss2,3

  • 1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massacheusetts Ave., Cambridge, MA, 02139, USA. yinqingl@mit.edu.

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2017
PubMed
Summary
This summary is machine-generated.

We developed a new DNA assembly framework for building complex genetic circuits efficiently. This modular system uses advanced recombination technologies and unique DNA sequences for reliable synthetic biology applications.

Keywords:
Gateway recombinationGene expressionGenetic circuitsGibson Assembl yHierarchical assemblyModular assemblySynthetic biology

More Related Videos

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.0K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Related Experiment Videos

Last Updated: Feb 24, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

13.0K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.0K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Area of Science:

  • Synthetic biology
  • Molecular biology
  • Genetic engineering

Background:

  • Efficient assembly of genetic circuits is crucial for advancing synthetic biology.
  • Current methods can be complex and require specialized equipment.

Purpose of the Study:

  • To present a novel assembly framework for constructing complex genetic circuits.
  • To integrate recent DNA recombination technologies for modular and reliable circuit construction.

Main Methods:

  • Utilized advanced DNA recombination technologies.
  • Incorporated unique nucleotide sequences for enhanced assembly.
  • Detailed protocols provided for implementation.

Main Results:

  • Demonstrated a modular and reliable framework for genetic circuit assembly.
  • Framework relies on readily available reagents and standard molecular biology techniques.
  • No specialized equipment is necessary for implementation.

Conclusions:

  • The presented framework enables efficient and reliable construction of complex genetic circuits.
  • This approach lowers the barrier to entry for synthetic biology research.
  • Facilitates the implementation of sophisticated gene expression systems.