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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Feb 24, 2026

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

Best practices for managing large CryoEM facilities.

Bart Alewijnse¹, Alun W Ashton², Melissa G Chambers³

¹NeCEN, Leiden University, Netherlands.

Journal of Structural Biology

|August 23, 2017

Summary

This summary is machine-generated.

This workshop overview highlights best practices for managing large cryo-electron microscopy (cryoEM) facilities, focusing on single-particle analysis workflows and user support. Key topics included sample handling, data processing, and training for cryoEM facility management.

Keywords:

Automation Cryotem Data management Facility management Single-particle User training

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Last Updated: Feb 24, 2026

gP2S, an Information Management System for CryoEM Experiments

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Published on: June 10, 2021

Electron Cryotomography of Bacterial Cells

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Published on: May 6, 2010

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Published on: March 16, 2022

Area of Science:

Structural Biology
Biophysics
Microscopy

Background:

The Workshop on the Management of Large CryoEM Facilities convened to address operational challenges and establish best practices.
Focus was placed on the burgeoning field of single-particle cryo-electron microscopy (cryoEM) and its facility management needs.

Framework:

The workshop established a framework for managing cryoEM facilities, emphasizing efficient user access and project assessment.
Discussions centered on optimizing cryoEM workflows from sample preparation to data analysis.

Implementation:

Key implementation areas included standardized sample handling protocols and advanced microscopy techniques.
Effective data management and processing strategies were critical components of the discussed workflows.

Implications:

The insights gained provide a roadmap for enhancing the operational efficiency and user support of large-scale cryoEM facilities.
Improved management practices are crucial for advancing structural biology research through cryoEM.