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Updated: Feb 24, 2026

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

Surfactant-free Colloidal Particles with Specific Binding Affinity.

Casper van der Wel¹, Nelli Bossert¹, Quinten J Mank¹

¹Biological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University , P.O. Box 9504, 2300 RA Leiden, The Netherlands.

Langmuir : the ACS Journal of Surfaces and Colloids

|August 30, 2017

Summary

This summary is machine-generated.

This study introduces a novel surfactant-free coating for colloidal particles, enhancing biosensing and drug delivery. The method ensures high binding affinity and stability in physiological conditions, crucial for targeting molecules in lipid membranes.

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Extraction and Characterization of Surfactants from Atmospheric Aerosols

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Published on: April 21, 2017

Area of Science:

Materials Science
Biotechnology
Surface Chemistry

Background:

Colloidal particles require specific surface functionalization for applications like biosensing and drug delivery.
Current particle coatings use surfactants that can desorb and negatively impact lipid membranes.
Developing stable, high-affinity particles for lipid membrane targeting necessitates alternative coating strategies.

Purpose of the Study:

To develop a generic, surfactant-free coating method for colloidal particles.
To achieve high binding affinity and colloidal stability under physiological conditions.
To create particles capable of targeting specific molecules within lipid membranes.

Main Methods:

Surface activation of polystyrene microparticles using EDC/Sulfo-NHS.
Sequential coating with a specific protein followed by covalent attachment of poly(ethylene) glycol (PEG).
Characterization of particle binding specificity using NeutrAvidin-biotin and Concanavalin A-cell interactions.

Main Results:

The developed method yields surfactant-free particles with high protein affinity and colloidal stability.
NeutrAvidin-functionalized particles demonstrated specific binding to biotinylated membranes.
Concanavalin A-functionalized particles showed specific binding to Dictyostelium discoideum cell glycocortex.
Particle affinity is tunable by adjusting protein density during the coating process.

Conclusions:

The novel coating method effectively transfers protein-specific affinity onto polystyrene microparticles.
This approach provides a stable and specific platform for targeting molecules in lipid membranes, overcoming limitations of surfactant-based coatings.
The generic nature of the method allows for broad applicability in biosensing, drug delivery, and self-assembly applications.