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Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Updated: Feb 23, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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Corrigendum: Isotope analysis in the transmission electron microscope.

Toma Susi, Christoph Hofer, Giacomo Argentero

    Nature Communications
    |August 31, 2017
    PubMed
    Summary
    This summary is machine-generated.

    This study corrects the article DOI for a previously published paper. Please refer to the updated DOI for accurate citation and access.

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    Area of Science:

    • Scientific Publishing
    • Bibliometrics
    • Scholarly Communication

    Background:

    • Accurate citation is crucial for scientific integrity.
    • Digital Object Identifiers (DOIs) ensure persistent access to research.
    • Correction notices are essential for maintaining the scholarly record.

    Purpose of the Study:

    • To provide the correct Digital Object Identifier (DOI) for a specific scientific article.
    • To ensure researchers can accurately locate and cite the intended publication.
    • To rectify a bibliographic error and maintain the integrity of scientific literature.

    Main Methods:

    • Identifying the incorrect DOI associated with the article.
    • Verifying the correct DOI through established scholarly databases.
    • Issuing a formal correction notice to alert the scientific community.

    Main Results:

    • The article DOI has been corrected to 10.1038/ncomms13040.
    • This correction ensures proper attribution and access to the research.
    • The scientific record is now updated with the accurate identifier.

    Conclusions:

    • The corrected DOI facilitates accurate referencing and retrieval of the scientific work.
    • This administrative correction upholds the standards of scholarly publishing.
    • Ensuring accurate metadata is vital for the discoverability and impact of research.