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Related Concept Videos

NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

3.4K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
3.4K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.4K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.4K
Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

3.6K
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
3.6K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.6K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.6K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.3K

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Updated: Feb 23, 2026

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
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Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

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Ultraclean pure shift NMR.

Pinelopi Moutzouri1, Yingxian Chen, Mohammadali Foroozandeh

  • 1School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. g.a.morris@manchester.ac.uk.

Chemical Communications (Cambridge, England)
|August 31, 2017
PubMed
Summary
This summary is machine-generated.

New pure shift NMR techniques eliminate periodic artefacts, enabling ultraclean spectra. This breakthrough allows for the detailed study of dilute mixture components previously obscured by spectral noise.

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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Spectroscopic Method Development

Background:

  • Proton NMR spectroscopy is crucial for chemical analysis.
  • Pure shift NMR techniques enhance spectral resolution by suppressing broadband proton signals.
  • Existing pure shift methods suffer from periodic artefacts, limiting their application for analyzing low-concentration analytes.

Purpose of the Study:

  • To develop a novel method for suppressing periodic artefacts in pure shift NMR spectra.
  • To enable the acquisition of ultraclean spectra for improved analysis of complex mixtures.
  • To present a technique compatible with all existing pure shift NMR methods.

Main Methods:

  • Development of a new pulse sequence and data processing strategy.
  • Integration of the technique with established pure shift NMR protocols.
  • Suppression of sidebands to arbitrary order.

Main Results:

  • Demonstrated effective suppression of periodic artefacts in pure shift NMR spectra.
  • Achieved ultraclean spectra, significantly improving signal-to-noise ratio.
  • Validated compatibility with various existing pure shift methodologies.

Conclusions:

  • The presented technique offers a robust solution for artefact removal in pure shift NMR.
  • This advancement broadens the applicability of pure shift NMR for analyzing dilute samples.
  • Ultraclean spectra facilitate more accurate and sensitive chemical structure elucidation.