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Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Related Experiment Video

Updated: Feb 23, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

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Using Phage Display to Create Recombinant Antibodies.

James R Dasch, Amy L Dasch

    Cold Spring Harbor Protocols
    |September 3, 2017
    PubMed
    Summary
    This summary is machine-generated.

    This study details a method for creating antibody phage display libraries using phagemid vectors. The protocol involves modifying vectors and using overlap extension PCR to generate antibody gene fusions for display on filamentous phage.

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    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Immunology

    Background:

    • Phage display technology is crucial for antibody engineering.
    • Current methods often utilize filamentous phage M13 or fd.
    • Antibody gene sequences are typically displayed on the phage minor coat protein pIII.

    Purpose of the Study:

    • To describe a protocol for constructing a phagemid library for antibody display.
    • To detail modifications of phagemid vectors for enhanced antibody display.
    • To enable the generation of diverse antibody libraries for screening.

    Main Methods:

    • Modification of the pBluescript KS(-) phagemid vector by replacing the multiple cloning site (MCS).
    • Utilizing overlap extension PCR to fuse antibody sequences with the M13 gene III fragment.
    • Construction of helper plasmids (pLINK and pFABC) for antibody fragment display.
    • Ligation of antibody cDNA libraries into the modified phagemid vector (pPDS).
    • Electroporation of the phagemid library into *Escherichia coli* with VCS-M13 helper phage.

    Main Results:

    • A novel phagemid vector (pPDS) was created with a modified MCS.
    • Helper plasmids were constructed to facilitate antibody fragment display.
    • A protocol for generating antibody cDNA libraries for phage display was established.

    Conclusions:

    • The described method provides a robust approach for creating antibody phage display libraries.
    • This protocol facilitates the generation and selection of antibodies with desired binding properties.
    • The methodology is applicable for constructing diverse antibody repertoires for various applications.