Core-Shell-Shell Multifunctional Nanoplatform for Intracellular Tumor-Related mRNAs Imaging and Near-Infrared Light...

Area of Science:

• Biomedical Engineering
• Nanoscience
• Oncology

Background:

• Multifunctional nanoplatforms integrating biosensing, imaging, and therapy are crucial for advanced biomedicine.
• Developing targeted cancer therapies with diagnostic capabilities remains a significant challenge.

Purpose of the Study:

• To create a core-shell-shell polydopamine (PDA)-modified upconversion nanoplatform for intracellular tumor mRNA detection.
• To achieve near-infrared (NIR) light-triggered synergistic photodynamic and photothermal therapy (PDT-PTT).

Main Methods:

• Fabrication of a UCNP@SiO2-MB@PDA core-shell-shell nanoconstruct.
• Development of a nanoprobe (UCNP@SiO2-MB@PDA-hpDNA) for mRNA sensing via DNA hybridization and PDA interactions.
• Utilizing upconversion fluorescence resonance energy transfer (FRET) for PDT and PDA's photothermal properties for PTT.

Main Results:

• The nanoprobe successfully detected intracellular tumor-related mRNAs and differentiated cancer cells.
• The nanoplatform demonstrated effective synergistic PDT-PTT upon NIR irradiation.
• The developed system integrated diagnostic and therapeutic functions within a single nanoconstruct.

Conclusions:

• The multifunctional nanoplatform shows significant promise for future oncotherapy.
• The integrated approach of cancer diagnosis and synergistic therapy offers a novel strategy.
• This nanoplatform advances the field of targeted cancer treatment and diagnostics.