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Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Related Experiment Video

Updated: Feb 23, 2026

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
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[Program optimization before enucleation on ovine somatic cell nuclear transfer].

Yanhua Guo1, Yiyuan Zhang1, Limin Wang1

  • 1Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi 832000, Xinjiang, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|September 7, 2017
PubMed
Summary
This summary is machine-generated.

Optimizing procedures before oocyte enucleation significantly improves ovine somatic cell nuclear transfer (SCNT) efficiency. This research enhances cloned embryo quality and quantity, leading to successful cloned sheep offspring.

Keywords:
adherent rateclone sheep offspringenuclation timeexposure duration of ovariesnuclear transfer

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Area of Science:

  • Animal Science
  • Reproductive Biology
  • Biotechnology

Background:

  • Ovine somatic cell nuclear transfer (SCNT) has limitations in efficiency.
  • Improving SCNT requires optimization of pre-enucleation procedures.

Purpose of the Study:

  • To optimize pre-enucleation protocols for ovine SCNT.
  • To enhance the efficiency and quality of cloned embryo production.

Main Methods:

  • Experiments involved varying ovary exposure duration, oocyte maturation time, and donor cell adherence rates.
  • Maturation, fusion, and cleavage rates of cloned embryos were assessed.
  • Microsatellite analysis confirmed the genetic identity of cloned fetuses.

Main Results:

  • Ovary exposure to 3 hours yielded higher oocyte maturation rates (60.18%) than 3-5 hours.
  • Oocyte maturation rates were significantly higher at 24 hours (89.06%) compared to 18 hours (53.81%).
  • Higher donor cell adherence (30%) improved fusion rates, and optimized maturation (18 hours) led to higher cleavage rates and successful cloned fetuses.

Conclusions:

  • Pre-enucleation program optimization is crucial for improving ovine SCNT.
  • Optimized protocols enhance the quantity and quality of cloned embryos.
  • Successful cloned sheep offspring were obtained through optimized SCNT schemes.