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$X-ray Diffraction of Biological Samples$ 01:10

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Updated: Feb 23, 2026

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Protein structure determination by electron diffraction using a single three-dimensional nanocrystal.

M T B Clabbers¹, E van Genderen², W Wan³

¹Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, Basel University, Mattenstrasse 26, CH-4058 Basel, Switzerland.

Acta Crystallographica. Section D, Structural Biology

|September 7, 2017

Summary

This summary is machine-generated.

Electron crystallography determined the structure of a rare lysozyme polymorph from a single nanocrystal, significantly advancing macromolecular structure determination for small crystals.

Keywords:

electron crystallography hybrid pixel detector protein nanocrystals

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Protein Crystallization for X-ray Crystallography

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

Area of Science:

Structural Biology
Biophysics
Crystallography

Background:

Macromolecular structure determination is often limited by crystal size.
Single-crystal X-ray crystallography struggles with nanometre-sized crystals.

Purpose of the Study:

To determine the structure of a rare dimeric polymorph of hen egg-white lysozyme.
To demonstrate the capability of electron crystallography for small macromolecular crystals.

Main Methods:

Electron crystallography using a Titan Krios transmission electron microscope.
Rotation method with parallel beam diffraction.
Low-dose diffraction data collection with a Timepix hybrid pixel detector.

Main Results:

Structure elucidation of a rare dimeric lysozyme polymorph from a single nanocrystal (0.14 µm³).
Achieved structure determination from a crystal an order of magnitude smaller than previously possible.
Demonstrated side-chain density and reconstruction using molecular replacement and automated model building.

Conclusions:

Electron crystallography is a viable alternative for determining biological structures from very small crystals.
This advancement significantly expands the scope of crystallographic structure determination.
The novel detector technology enhances low-dose electron diffraction data collection.