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All-Aqueous Assemblies via Interfacial Complexation: Toward Artificial Cell and Microniche Development.

Sarah D Hann1, Kathleen J Stebe1, Daeyeon Lee1

  • 1Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.

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Summary

Artificial structures using aqueous two-phase systems (ATPSs) mimic natural cellular confinement. These systems enable tailored structure formation and modulation for synthetic cells and specialized studies.

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Area of Science:

  • Biomolecular and cellular confinement studies.
  • Synthetic biology and artificial cell development.

Background:

  • Natural environments, including cellular compartments, regulate biomolecule and cell structure, function, and properties through confinement.
  • Membraneless organelles and cellular functions are influenced by internal and external confinement effects.
  • Aqueous two-phase systems (ATPSs) offer a promising platform to mimic multiscale confinement.

Purpose of the Study:

  • To highlight recent advancements in creating and stabilizing ATPS-droplet-based systems.
  • To focus on the role of interfacial complexation in these systems.
  • To discuss the potential of ATPSs for tailored structure formation and confinement studies.

Main Methods:

  • Development and stabilization of aqueous two-phase system (ATPS) droplets.
  • Utilizing interfacial complexation for structure formation and modulation.
  • Designing systems for triggered assembly and disassembly.

Main Results:

  • ATPS-droplet systems can be produced and stabilized effectively.
  • Interfacial complexation allows for controlled structure formation and modulation.
  • These systems enable tailored structures for specific functions and confinement studies.

Conclusions:

  • ATPS-droplet systems are versatile tools for recapitulating biological confinement.
  • Interfacial complexation is key to controlling structure formation and dynamics.
  • Further research is needed for synthetic cell applications and niche confinement studies.