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High-Performance Liquid Chromatography (HPLC)-Based Detection and Quantitation of Cellular c-di-GMP
1Department of Biological Sciences, Binghamton Biofilm Research Center (BBRC), Binghamton University, 2401 ITC Building, 85 Murray Hill Road, Binghamton, NY, 13902, USA. opetrov0@binghamton.edu.
Abstract:
The modulation of c-di-GMP levels plays a vital role in the regulation of various processes in a wide array of bacterial species. Thus, investigation of c-di-GMP regulation requires reliable methods for the assessment of c-di-GMP levels and turnover. Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from Pseudomonas aeruginosa samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.
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