Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amperometric enzyme electrodes.

W J Albery1, P N Bartlett, A E Cass

  • 1Department of Chemistry, Imperial College of Science and Technology, London, U.K.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|August 28, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Specifically horizontally tethered DNA probes on Au surfaces allow labelled and label-free DNA detection using SERS and electrochemically driven melting.

Chemical science·2018
Same author

Strain discrimination of <i>Yersinia pestis</i> using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences.

Chemical science·2018
Same author

Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of immobilized enzyme biosensors: Comparison with experiments.

Journal of electroanalytical chemistry (Lausanne, Switzerland)·2015
Same author

Electrodeposition from supercritical fluids.

Physical chemistry chemical physics : PCCP·2014
Same author

Influence of macroporous gold support and its functionalization on lactate oxidase-based biosensors response.

Talanta·2012
Same author

Enhancing solar cells with localized plasmons in nanovoids.

Optics express·2011
Same journal

The microlandscapes of tree trunks: the effect of lichen and tree-level characteristics on arthropod communities.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Centimetre-scale landscapes to assess the motion behaviour and cognition of gastropods and bivalves.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Intertidal microcosms of wave-swept rocky shores: ecological and physiological insights from a uniquely stressful environment.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Temporal and spatial variation in temperature and oxygen at the microscale: key niche axes for aquatic life.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Natural microcosms in ecology: fulfilling the promise of model systems?

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Microbe-induced galls and plant defence: metabolite crosstalk in a co-evolutionary battle.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
See all related articles

This study presents novel amperometric enzyme electrodes for detecting ethanol, bile acids, and glucose. It also details sensors for toxic gases like H2S and HCN using enzyme inhibition.

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Biosensors

Background:

  • Amperometric enzyme electrodes are crucial for biochemical analysis.
  • Developing efficient and selective biosensors is an ongoing challenge.
  • Existing methods may require membranes or lack in vivo applicability.

Purpose of the Study:

  • To describe three distinct types of amperometric enzyme electrodes.
  • To demonstrate their application in detecting various analytes, including biomolecules and toxic gases.
  • To investigate the underlying mechanisms of enzyme oxidation and sensor performance.

Main Methods:

  • Utilizing conducting organic-salt electrodes for enzyme immobilization and redox reactions.
  • Developing sensors for NADH oxidation, ethanol, bile acids, and flavoenzymes.

Related Experiment Videos

  • Investigating heterogeneous redox catalysis for enzyme oxidation.
  • Designing sensors for in vivo glucose monitoring in rats.
  • Employing enzyme inhibition of cytochrome oxidase for toxic gas detection (H2S, HCN).

    • Main Results:

    • Successful development of sensors for ethanol and bile acids.
    • Demonstration of direct oxidation of five different flavoenzymes on the electrode surface.
    • Confirmation of heterogeneous redox catalysis and strong enzyme adsorption, enabling membrane-less microelectrodes.
    • Real-time in vivo monitoring of glucose levels in rat brains.
    • Detection of low concentrations of H2S and HCN via enzyme inhibition.

    Conclusions:

    • Conducting organic-salt electrodes offer a versatile platform for amperometric enzyme biosensors.
    • The developed sensors show promise for both in vitro biochemical analysis and in vivo monitoring.
    • Enzyme inhibition provides a sensitive method for detecting toxic gases.