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Related Concept Videos

¹³C NMR: ¹H–¹³C Decoupling01:04

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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Related Experiment Video

Updated: Feb 23, 2026

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Optimized decoupling schemes in ultrafast HSQC experiments.

Laetitia Rouger1, Serge Akoka1, Patrick Giraudeau2

  • 1CNRS UMR 6230 CEISAM, Université de Nantes, Nantes, France.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 13, 2017
PubMed
Summary
This summary is machine-generated.

Ultrafast 2D NMR (Nuclear Magnetic Resonance) experiments can now achieve full spectral range without compromising resolution. New continuous decoupling schemes using adiabatic pulses minimize artifacts in ultrafast HSQC experiments.

Keywords:
Adiabatic decouplingHSQCInterleaving artifactsUltrafast NMR

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Biophysical Chemistry

Background:

  • Ultrafast (UF) 2D NMR enables rapid data acquisition within a single scan.
  • Current UF-HSQC experiments face limitations in balancing spectral width and resolution.
  • Interleaved acquisitions, while extending spectral range, introduce significant artifacts.

Purpose of the Study:

  • To investigate the cause of artifacts in UF-HSQC experiments.
  • To develop improved decoupling schemes for UF-HSQC.
  • To enable acquisition of full-range UF-HSQC spectra without compromising resolution.

Main Methods:

  • Optimization of four continuous decoupling schemes utilizing adiabatic pulses.
  • Application of these schemes to UF-HSQC experiments.
  • Analysis of spectral quality and artifact reduction.

Main Results:

  • Artifacts in UF-HSQC are primarily linked to discontinuous decoupling.
  • Optimized continuous adiabatic decoupling schemes significantly reduce artifacts.
  • Full-range UF-HSQC spectra are accessible with enhanced resolution and quality.

Conclusions:

  • Continuous decoupling schemes with adiabatic pulses are effective in overcoming limitations of UF-HSQC.
  • This advancement allows for broader application of UF-NMR techniques.
  • Improved spectral quality enhances the utility of UF-HSQC in various scientific fields.