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Development of a plug and play ImmunoPCR technique for the analysis of biomolecules
Sreeja Gopal1, Shobha Purushothama1, Alvydas Mikulskis1
1Global Biomarker Discovery & Development, Biogen Inc, Cambridge, MA 02142, USA.
Aim:
ImmunoPCR technology combines the advantages of specificity and robustness of a ligand binding assay with the amplification potential of PCR. We describe through three case studies a plug-and-play immuno polymerase chain reaction (iPCR) technique to measure biomolecules.
Results:
Case Study 1 demonstrated feasibility of measurement of IgG1 in cerebrospinal fluid at the desired level of sensitivity with minimal cost and timelines of clinical assay implementation. Case Study 2 translated the iPCR protocol to measure multiple IgG1 analytes in cerebrospinal fluid. Case Study 3 demonstrated broad applicability of the technique to yet another analyte IL-6.
Conclusion:
The advantages of our iPCR approach were: lack of reliance on a single vendor for technology platform/software, minimal reliance on proprietary reagents and reduced method development times and cost.
