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Updated: Feb 23, 2026

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
A genetically encoded cyclobutene probe for labelling of live cells
1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA. pdussault1@unl.edu jguo4@unl.edu.
Abstract:
We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.
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