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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Published on: September 26, 2013
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Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells
Dominik Duscher1, Zeshaan N Maan2, Anna Luan2
1Division of Plastic Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA; Department of Plastic and Hand Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
Cytotherapy
|September 18, 2017
Summary
Ultrasound-assisted liposuction (UAL) safely harvests adipose-derived mesenchymal stromal cells (ASCs) for regenerative medicine. Both VASER and Lysonix 3000 UAL methods yield functional ASCs that enhance tissue healing and blood vessel formation.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Biomaterials Science
Background:
- Mesenchymal stromal cells (MSCs), particularly adipose-derived mesenchymal stromal cells (ASCs), are crucial for regenerative medicine due to their plasticity and regenerative properties.
- Liposuction offers an accessible method for harvesting ASCs, but different liposuction techniques may impact cell functionality.
- The impact of popular ultrasound-assisted liposuction (UAL) devices, VASER and Lysonix 3000, on ASCs requires thorough investigation.
Purpose of the Study:
- To assess the impact of two leading ultrasound-assisted liposuction (UAL) devices, VASER and Lysonix 3000, on the functionality of adipose-derived mesenchymal stromal cells (ASCs).
- To evaluate ASC yield, viability, differentiation potential, and in vivo regenerative performance after harvest using different UAL methods.
Main Methods:
- Adipose-derived mesenchymal stromal cells (ASCs) were isolated from lipoaspirate using fluorescent-assisted cell sorting based on CD34+, CD31-, and CD45- markers.
- ASC yield, viability, osteogenic and adipogenic differentiation capacities were evaluated.
- In vivo regenerative performance, including soft tissue healing and neovascularization, was assessed using UAL-derived ASCs.
Main Results:
- Both VASER and Lysonix 3000 UAL methods yielded equivalent ASCs in terms of quantity and viability.
- ASCs from VASER UAL exhibited enhanced osteogenic and adipogenic marker expression, though overall differentiation capacity was comparable.
- Both UAL-derived ASCs significantly improved soft tissue healing and neovascularization in vivo, with no significant differences between the two UAL groups.
Conclusions:
- Ultrasound-assisted liposuction (UAL) provides a safe and efficient method for harvesting mesenchymal stromal cells from adipose tissue.
- ASCs obtained through UAL are suitable for applications in cell therapy and tissue engineering.
- UAL is a viable technique for obtaining functional ASCs for regenerative medicine purposes.
