Related Experiment Video
Updated: Feb 22, 2026

Picometer-Precision Atomic Position Tracking through Electron Microscopy
Published on: July 3, 2021
Three-Dimensional (3D) Nanometrology Based on Scanning Electron Microscope (SEM) Stereophotogrammetry.
Vipin N Tondare1,2, John S Villarrubia1, András E Vladár1
1Engineering Physics Division, Physical Measurement Laboratory, National Institute of Standards and Technology (NIST), 100 Bureau Drive, Stop 8212, Gaithersburg, MD 20899, USA.
Stereophotogrammetry software packages for 3D surface reconstruction from scanning electron microscope (SEM) images show high error in uniform zones. Further improvements are needed for reliable 3D modeling of low-contrast SEM samples.
Area of Science:
- Materials Science
- Computer Vision
- Metrology
Background:
- Three-dimensional (3D) surface reconstruction from Scanning Electron Microscope (SEM) images is a long-established technique.
- Several commercial stereophotogrammetry software packages are currently available for this purpose.
Purpose of the Study:
- To evaluate the performance of commercially available stereophotogrammetry software packages.
- To assess the accuracy of 3D reconstruction using simulated SEM images of virtual samples with known dimensions.
Main Methods:
- Utilized Monte Carlo simulated SEM images of virtual samples with precisely known geometry.
- Tested two stereophotogrammetry software packages by comparing their reconstructed 3D models against the ground truth of the virtual samples.
- Investigated performance on samples with both rough and uniform (low-contrast) surfaces.
Main Results:
- Both tested software packages performed well on simulated SEM images of samples with rough surfaces.
- Significant height reconstruction errors (approximately 46%) were observed in samples with nearly uniform, low-contrast zones.
- The accuracy of 3D reconstruction is highly dependent on surface topography and contrast.
Conclusions:
- Current stereophotogrammetry software packages require further development for reliable 3D reconstruction from SEM images, particularly those with uniform or low-contrast regions.
- The use of simulated SEM images provides a valuable method for algorithm testing and validation in 3D reconstruction.
More Related Videos
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Overview of Microscopy Techniques

