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Objective assessment of CNS function within 6 hours of spinal cord ischemia in rabbits
Tadaomi-Alfonso Miyamoto1, Koho-Julio Miyamoto2, Nobuhisa Ohno3
1Research Department, Kokura Memorial Hospital, 1-1 Kifunecho, Kokurakita-ku, 802, Kitakyushu-shi, Japan.
Purpose:
To develop a neurologic scoring (NS) system to objectively assess CNS function shortly after spinal cord ischemia.
Methods:
Spinal cord ischemia was induced by temporarily clamping the infrarenal aorta in 27 rabbits anesthetized with isoflurane/N2O/O2 without muscle relaxants. Animals were divided ito group I, normothermic ischemia [I-a, 11 min (n=8); I-b, 12 min (n=8)], and group II, 60 min hypothermic ischemia targeted to II-a, 29.5°C (n=5), and II-i, 30.0°C (n=6). Postischemic neurologic function was scored from 0 to 6.
Results:
Seventy-five percent of each group I subgroup ended with paraplegia. Function in the I-b group tended to be worse than in I-a (NS=1.7vs 1.9P>0.05). Hypothermia of 29.9±0.1°C protected partially (NS=2.8), whereas 29.4±0.1°C resulted in significantly higher NS, starting at 150 min (P<0.05vs IIi) with total recovery 5.5 hours (P<0.0001) post re-perfusion.
Conclusions:
Protection of the spinal cord from ischemia can be objectively quantitated by our system. Protection strategies can be compared within 6 h of the ischemia-insult.
