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Updated: Feb 22, 2026

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Live-Cell STED Imaging with the HyPer2 Biosensor.

Natalia M Mishina¹, Vsevolod V Belousov²

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow, 117997, Russia.

Methods in Molecular Biology (Clifton, N.J.)

|September 20, 2017

Summary

This summary is machine-generated.

Stimulated emission depletion (STED) microscopy enables super-resolution imaging of cellular structures. This study details a protocol for live-cell STED imaging using the HyPer2 biosensor fused to cytoskeletal filaments for functional insights.

Keywords:

Biosensors HyPer2 Hydrogen peroxide Live-cell imaging STED Super-resolution microscopy

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Last Updated: Feb 22, 2026

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

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Published on: June 2, 2023

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Stimulated emission depletion (STED) microscopy is a key super-resolution technique.
Genetically encoded biosensors offer advantages over synthetic dyes and fluorescent proteins for STED imaging.
Fusion of biosensors with target proteins allows simultaneous imaging and functional analysis of cellular components.

Purpose of the Study:

To establish a protocol for live-cell STED microscopy.
To demonstrate the application of the HyPer2 biosensor fused to cytoskeletal filaments.
To enable subdiffraction imaging of intracellular macromolecular architecture and cellular activities.

Main Methods:

Live-cell STED microscopy protocol development.
Genetic fusion of the HyPer2 biosensor with cytoskeletal filament proteins.
Super-resolution imaging of cellular structures and dynamics.

Main Results:

Successful implementation of live-cell STED microscopy for biosensor imaging.
Visualization of cytoskeletal filaments at subdiffraction resolution.
Acquisition of functional information regarding cellular activities through biosensor reporting.

Conclusions:

Genetically encoded biosensors are effective for STED microscopy.
The developed protocol allows for high-resolution imaging of cellular architecture and function.
This approach provides new avenues for studying dynamic cellular processes in living cells.