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Updated: Feb 22, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
Published on: October 4, 2012
Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)
Mário R Santos1, Cláudia Bispo1,2, Jörg D Becker3
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande Nº6, 2780-156, Oeiras, Portugal.
Abstract:
Efficient methods to isolate highly purified Arabidopsis thaliana pollen and the subcellular components of the male gametophyte (the vegetative nucleus and two sperm cells) have enabled genome-scale studies revealing a highly dynamic reprogramming of the transcriptome and epigenome during pollen development. Here, we describe the isolation of uninucleate microspores, mature pollen, as well as sperm cells and vegetative nuclei by Fluorescence-Activated Cell Sorting.

