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[Manipulation of cofactor balance in microorganisms]
Xiulai Chen1,2,3, Jia Liu1,2,3, Qiuling Luo1,2,3
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|September 30, 2017
Summary
Maintaining cofactor balance is crucial for industrial microbial production of enzymes, pharmaceuticals, and chemicals. Strategies for biochemical and metabolic engineering can optimize this balance for maximal carbon flux and consistent cofactor levels.
Area of Science:
- Microbial physiology and biotechnology
- Metabolic engineering and synthetic biology
Background:
- Cofactor balance is essential for efficient microbial biosynthesis of valuable compounds.
- Industrial production demands stable metabolic pathways and consistent cofactor levels in microorganisms.
Purpose of the Study:
- To review the physiological roles of cofactors in microbial metabolism.
- To discuss strategies for manipulating cofactor balance using engineering approaches.
- To identify future research directions for improved cofactor regulation.
Main Methods:
- Literature review of cofactor physiology and metabolic engineering strategies.
- Analysis of biochemical engineering techniques for cofactor manipulation.
- Discussion of metabolic engineering approaches to control cofactor homeostasis.
Main Results:
- Cofactors are critical for numerous enzymatic reactions, directly impacting metabolic flux.
- Biochemical and metabolic engineering offer viable strategies to modulate cofactor levels.
- Current methods allow for targeted adjustments in cofactor pools to enhance production.
Conclusions:
- Optimizing cofactor balance is key to maximizing carbon flux towards target products in industrial microbes.
- Further research into advanced engineering techniques is needed to refine cofactor regulation for robust bioproduction.
- Effective cofactor management is fundamental for sustainable and efficient biotechnological processes.
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