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Updated: Feb 22, 2026

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
Published on: December 5, 2025
Characterization of GPCRs in extracellular vesicle (EV)
Manoj R Medapati1, Anula Singh2, Ranjit R Korupally2
1College of Dentistry, Rady Faculty of Health of Sciences, Winnipeg, MB, Canada; Manitoba Chemosensory Biology (MCSB) Research Group, University of Manitoba, Winnipeg, MB, Canada.
Abstract:
Extracellular vesicle (EV) are tiny membranous vesicles usually <500nm in size that recently emerged as a new paradigm in human intercellular signaling. EVs have shown a promising role in development of diagnostic markers in many pathophysiological disorders. The presence of chemosensory and therapeutically relevant G protein-coupled receptors (GPCRs) on EV membranes is poorly characterized. Here, we compare different methods including ultracentrifugation and polymer-charge-based separation to isolate EVs from cell culture media and human saliva. The presence of bitter taste GPCRs (T2R4 and T2R38) and a class A GPCR angiotensin II type 1 receptor on these EVs was characterized by qPCR, ELISA, and immunotransmission electron microscopy.
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