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Chromosomal mapping of the structural gene coding for the mouse cell adhesion molecule uvomorulin
H R Eistetter1, S Adolph, M Ringwald
1Friedrich-Miescher-Laboratorium, Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
Summary
The mouse uvomorulin gene (Um) was mapped to chromosome 8 using genetic crosses and in situ hybridization. This finding provides a new marker for the mouse genetic map.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Uvomorulin is a cell adhesion molecule crucial for embryonic development.
- Understanding the genetic basis and chromosomal location of key developmental genes is essential for mapping genetic traits.
Purpose of the Study:
- To determine the chromosomal location of the mouse uvomorulin gene.
- To establish the precise position of the uvomorulin locus on the mouse genetic map.
Main Methods:
- Restriction fragment length polymorphism (RFLP) analysis of mouse genomic DNA.
- Interspecific backcross analysis to track gene segregation.
- In situ hybridization to metaphase chromosomes.
Main Results:
- The uvomorulin gene (Um) was mapped to mouse chromosome 8.
- The Um locus was localized approximately 14 centimorgans (cM) distal to serum esterase 1 (Es-1) and 5 cM proximal to tyrosine aminotransferase (Tat).
- In situ hybridization confirmed the Um locus is near the distal end of chromosome 8 (bands C3-E1).
Conclusions:
- The chromosomal assignment of the uvomorulin gene provides a valuable new marker for the mouse genetic map.
- This localization aids in understanding the organization and evolution of chromosome 8.