Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.6K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.7K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.7K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

3.9K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.9K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.3K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.3K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

3.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain.

Current biology : CB·2025
Same author

Author Correction: Endophilin marks and controls a clathrin-independent endocytic pathway.

Nature·2025
Same author

A single-particle analysis method for detecting membrane remodelling and curvature sensing.

Journal of cell science·2024
Same author

Adaptations of membrane trafficking in cancer and tumorigenesis.

Journal of cell science·2024
Same author

The E3 ubiquitin ligase Itch regulates death receptor and cholesterol trafficking to affect TRAIL-mediated apoptosis.

Cell death & disease·2024
Same author

Margarete Heck (1959-2023): Cell biologist, geneticist, and incandescent social spark.

The Journal of cell biology·2023
Same journal

The Power of Nup85: Controlling Molecular Traffic and Cellular Fate.

Traffic (Copenhagen, Denmark)·2026
Same journal

Traffic Light Commentary-Src in the Upside Down: A Kinase Turned Inside Out.

Traffic (Copenhagen, Denmark)·2026
Same journal

Integrating Lateral Super-Resolution and Axial Progression Reveals Distinct Clathrin Pit Formation Pathways.

Traffic (Copenhagen, Denmark)·2026
Same journal

A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.

Traffic (Copenhagen, Denmark)·2026
Same journal

Mechanistic Insight Into Clathrin-Mediated Endocytosis in Plants.

Traffic (Copenhagen, Denmark)·2026
Same journal

Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.

Traffic (Copenhagen, Denmark)·2026
See all related articles

Related Experiment Video

Updated: Feb 21, 2026

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
08:11

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons

Published on: June 19, 2016

11.2K

Eps15R and clathrin regulate EphB2-mediated cell repulsion.

Emma Evergren1,2, Neville Cobbe2, Harvey T McMahon1

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

Traffic (Copenhagen, Denmark)
|October 4, 2017
PubMed
Summary
This summary is machine-generated.

EphB/ephrinB cell repulsion relies on trans-endocytosis, a process mediated by clathrin and Eps15R. This study reveals Eps15R

Keywords:
EphB2Eps15Eps15RNumbclathrintrans-endocytosis

More Related Videos

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

81.0K
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
10:21

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

6.6K

Related Experiment Videos

Last Updated: Feb 21, 2026

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
08:11

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons

Published on: June 19, 2016

11.2K
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

81.0K
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
10:21

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

6.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Eph receptors and ephrin ligands mediate cell-cell interactions crucial for tissue development.
  • EphB/ephrinB interactions drive cell repulsion, requiring trans-endocytosis of receptor-ligand complexes.
  • The molecular mechanisms governing this trans-endocytosis are not well understood.

Purpose of the Study:

  • To elucidate the molecular mechanism of EphB/ephrinB trans-endocytosis.
  • To investigate the roles of clathrin and Eps15R in mediating cell repulsion.

Main Methods:

  • Utilized Co115 colorectal cell lines stably expressing EphB2 and ephrinB1.
  • Performed knockdown experiments targeting Eps15R and Eps15.
  • Investigated in vitro binding between Eps15R and clathrin.
  • Conducted rescue experiments in EphB2 co-culture assays.

Main Results:

  • Knockdown of Eps15R significantly reduced EphB2/ephrinB-mediated cell repulsion.
  • Identified a novel Eps15R motif (DPFxxLDPF) that binds directly to clathrin.
  • Demonstrated that Eps15R-clathrin interaction is essential for EphB2-mediated cell repulsion.

Conclusions:

  • Ephrin receptor trans-endocytosis and subsequent cell repulsion are mediated by clathrin and Eps15R.
  • Eps15R acts as a crucial link between EphB/ephrinB complexes and the clathrin machinery.
  • This provides the first mechanistic insight into EphB/ephrinB trans-endocytosis.