IMMUNOHISTOCHEMICAL ANALYSIS OF APPENDIX CELL WALL INFILTRATE IN ACUTE PHLEGMONOUS APPENDICITIS

The purpse of the study was to analyze the content of the mucous infiltrate of cells of the lamina propria of the appendix using immunohistochemical analysis in patients with acute phlegmonous appendicitis. The research included 21 patients with acute phlegmonous appendicitis. The control group consisted of 15 persons lacking the inflammation of the intraperitoneal cavity. Primary monoclonal (MC) antibodies (AB) of the mouse used in the immunohistochemical (IHC) research included the following: Ki-67, CD3, CD4, CD8, CD20, CD45, CD45R0, and CD68 (DAKO, Denmark), as well as EnVision+ System-HRP (DAB) optical system. The research has shown that acute phlegmonous appendicitis is characterized by statistically reliable increased infiltration (p<0.001) by B-lymphocytes (CD20), memory T-cells (CD45R0), Ki-67+ cells, macrophages (CD68), T-lymphocytes (CD3), T-helper cells (CD4), cytotoxic T-lymphocytes (CD8), and CD45 cells of the lamina propria of the appendix when compared with the control group. Development of acute phlegmonous appendicitis is characterized by enhance infiltration of the lamina propria by B-lymphocytes (CD20), macrophages (CD68), memory T-cells, and high proliferation activity of the mononuclear cells (Ki-67 index).