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Updated: Feb 21, 2026

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
Published on: May 3, 2021
Effect of buffer at nanoscale molecular recognition interfaces - electrostatic binding of biological polyanions
Ana C Rodrigo1, Erik Laurini, Vânia M P Vieira
1Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. david.smith@york.ac.uk.
Abstract:
We investigate the impact of an over-looked component on molecular recognition in water-buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris-HCl > HEPES > PBS). The heparin binding of self-assembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.
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