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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: Feb 21, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Comparative DIGE Proteomics.

Kay Ohlendieck¹

¹Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland. kay.ohlendieck@mu.ie.

Methods in Molecular Biology (Clifton, N.J.)

|October 12, 2017

Summary

This summary is machine-generated.

Difference gel electrophoresis (DIGE) is a powerful tool for comparative proteomics, enabling systematic protein cataloging in various biological samples. Its pre-electrophoretic fluorescent labeling offers significant technical advantages for bioanalytical studies.

Keywords:

CyDye DIGE Difference gel electrophoresis Difference in-gel electrophoresis Fluorescence labeling Gel electrophoresis Mass spectrometry Proteomics Two-dimensional gel electrophoresis

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Proteomics
Biochemistry
Analytical Chemistry

Background:

Gel-based proteomics is essential for cataloging proteins in diverse biological specimens.
Comparative analyses of complex biological samples are crucial in biomedical research.
Electrophoretic techniques are fundamental in modern biochemical approaches.

Purpose of the Study:

To highlight the technical advantages of pre-electrophoretic fluorescent labeling.
To discuss the bioanalytical usefulness of difference gel electrophoresis (DIGE).
To emphasize DIGE as a powerful tool in comparative proteomics.

Main Methods:

Utilizing difference gel electrophoresis (DIGE) for large-scale protein separation.
Employing both 2-dye and 3-dye fluorescence systems.
Applying minimal or saturation labeling techniques.

Main Results:

DIGE has become the most powerful analytical tool for comparative proteomics.
Pre-electrophoretic fluorescent labeling offers distinct technical advantages.
The method is highly successful for bioanalytical applications.

Conclusions:

DIGE is a highly effective electrophoretic method for comparative proteomics.
The technique facilitates systematic protein cataloging and analysis.
Fluorescent labeling enhances the utility of DIGE in bioanalytical studies.