Related Experiment Video
Updated: Feb 20, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
2D-DIGE proteomics
Kay Ohlendieck1, Paul Dowling1
1Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland.
None:
Gel electrophoresis is a key technique of modern biochemistry and frequently used for efficient protein separation prior to top-down proteomics. For comparative studies, differential fluorescent tagging can be employed to avoid gel-to-gel variations, which has been extensively used in two-dimensional difference gel electrophoresis (2D-DIGE). This chapter reviews recent advances in 2D-DIGE and focuses on comparative proteomic studies. Strategies to improve the sensitivity of this gel-based method, as well as a discussion of its bioanalytical advantages versus technical limitations are provided. The general suitability of 2D-DIGE for the large-scale screening of cellular systems and biofluids to support biomarker discovery is explored.
More Related Videos
10:51Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Published on: April 10, 2014
12:34Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...