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Optical clearing for multiscale biological tissues.

Tingting Yu1,2, Yisong Qi1,2, Hui Gong1,2

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Journal of Biophotonics
|October 13, 2017
PubMed
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Tissue optical clearing enhances 3D imaging depth by reducing light scattering in biological tissues. This review guides researchers in selecting optimal clearing methods for diverse samples and imaging techniques.

Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Histology

Background:

  • 3D reconstruction of tissue is crucial for biomedical research.
  • Light scattering limits optical imaging depth in heterogeneous biological tissues.
  • Tissue optical clearing offers a solution for high-resolution, deep-tissue imaging.

Purpose of the Study:

  • To review in vitro tissue optical clearing techniques for end-users.
  • To categorize methods based on sample characteristics (size, age).
  • To identify methods compatible with immunostaining and lipophilic dyes, and various imaging modalities.

Main Methods:

  • Literature review of existing tissue optical clearing techniques.
  • Classification of methods by sample features (size, age).
Keywords:
biological tissuesoptical clearingoptical imagingsample featurestaining

Related Experiment Videos

  • Analysis of compatibility with specific labeling methods and imaging systems.

    • Main Results:

    • Various optical clearing methods exist, each with specific advantages.
    • Method selection depends on sample properties and experimental goals.
    • Combinations with different imaging techniques are feasible.

    Conclusions:

    • This review provides a user-centric guide to selecting appropriate tissue optical clearing methods.
    • Choosing the right method is key to successful deep-tissue imaging and 3D reconstruction.
    • The review facilitates informed decisions for researchers in biomedical imaging.