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Related Experiment Video

Updated: Feb 21, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

sideSPIM - selective plane illumination based on a conventional inverted microscope.

Per Niklas Hedde^1,2, Leonel Malacrida^1,3,4, Siavash Ahrar^5,6

¹Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.

Biomedical Optics Express

|October 14, 2017

Summary

This summary is machine-generated.

SideSPIM, a novel light sheet microscopy technique, enhances imaging performance and sample handling. This adaptable system integrates with existing microscopes for cost-effective, high-throughput 3D imaging.

Keywords:

(110.6880) Three-dimensional image acquisition (170.2520) Fluorescence microscopy (180.6900) Three-dimensional microscopy (220.1000) Aberration compensation (220.2945) Illumination design

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Area of Science:

Microscopy and Imaging Technologies
Biotechnology and Biomedical Engineering

Background:

Existing selective plane illumination microscopy (SPIM) methods often compromise ease of use or imaging performance.
There is a need for cost-effective, flexible light sheet microscopy adaptable to standard research microscopes.

Purpose of the Study:

To develop a novel light sheet microscopy approach, sideSPIM, that balances high imaging performance with user-friendly sample handling.
To enable new applications in fluidics and high-throughput 3D imaging of multiple specimens using plane illumination.

Main Methods:

Implementation of sideSPIM based on an inverted epifluorescence microscope with minimal system modifications.
Leveraging a sCMOS camera and piezo stage for rapid data acquisition (up to 5 stacks/s).
Ensuring compatibility with established sample handling methods and adaptable magnification for diverse fields of view.

Main Results:

SideSPIM achieves uncompromised imaging performance and simplifies sample manipulation.
The system supports high-throughput 3D imaging of multiple specimens and fluidic applications.
Data acquisition rates of up to 5 stacks/s were demonstrated.

Conclusions:

SideSPIM offers a flexible, cost-effective solution for advanced light sheet microscopy.
The technique maintains existing microscope functionality while enhancing imaging capabilities.
SideSPIM is suitable for a wide range of applications, from single cells to whole organisms, with easy scalability.