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Updated: Aug 19, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
Chikungunya fever outbreak identified in North Bali, Indonesia
Kartika Sari1, Khin Saw Aye Myint2, Ayu Rai Andayani3
1Faculty of Medicine and Health Sciences, Warmadewa University, Denpasar, Bali, Indonesia80239.
Background:
Chikungunya virus (CHIKV) infections have been reported sporadically within the last 5 years in several areas of Indonesia including Bali. Most of the reports, however, have lacked laboratory confirmation.
Method:
A recent fever outbreak in a village in the North Bali area was investigated using extensive viral diagnostic testing including both molecular and serological approaches.
Results And Conclusions:
Ten out of 15 acute febrile illness samples were confirmed to have CHIKV infection by real-time PCR or CHIKV-specific IgM enzyme-linked immunosorbent assay (ELISA). The outbreak strain belonged to the Asian genotype with highest homology to other CHIKV strains currently circulating in Indonesia. The results are of public health concern particularly because Bali is a popular tourist destination in Indonesia and thereby the potential to spread the virus to non-endemic areas is high.
Genbank Accession Numbers:
KY885022, KY885023, KY885024, KY885025, KY885026, KY885027.
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