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Customizing the spent coffee for Trichoderma reesei cellulase immobilization by modification with activating agents
Aneta Buntić1, Marija Pavlović1, Dušan Antonović1
1Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
International Journal of Biological Macromolecules
|October 17, 2017
Summary
This study repurposed waste coffee grounds into a porous carrier for cellulase enzyme immobilization. Chlorine dioxide treatment yielded the best results, enhancing enzyme productivity for industrial applications.
Area of Science:
- Biotechnology
- Materials Science
- Chemical Engineering
Background:
- Spent coffee grounds represent a significant waste stream with potential for valorization.
- Enzyme immobilization is crucial for enhancing enzyme stability, reusability, and industrial applicability.
- Developing cost-effective and sustainable carriers for enzyme immobilization is an ongoing challenge.
Purpose of the Study:
- To investigate the feasibility of using modified spent coffee grounds as a carrier for cellulase enzyme immobilization.
- To optimize the surface modification process for creating a suitable porous structure on coffee grounds.
- To evaluate the performance of immobilized cellulase in terms of yield and efficiency.
Main Methods:
- Surface modification of spent coffee grounds using activating agents like glutaraldehyde, chlorine dioxide, and hydrogen peroxide.
- Characterization of modified coffee grounds using Fourier-Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM).
- Immobilization of cellulase enzyme onto the modified coffee grounds and kinetic analysis using the pseudo-second-order model.
Main Results:
- Chlorine dioxide emerged as the most effective activating agent for coffee surface modification.
- Optimal immobilization conditions were achieved with a 30% chlorine dioxide solution for 30 minutes at a 6mL/g activator/carrier ratio.
- The highest immobilization yield reached 55%, with an immobilization efficiency of 45%.
- The immobilization process followed a pseudo-second-order kinetic model.
Conclusions:
- Spent coffee grounds can be effectively modified to serve as a sustainable and efficient carrier for cellulase immobilization.
- The developed method offers a promising approach for waste valorization and enhancing enzyme productivity in industrial processes.
- This research opens new avenues for utilizing agricultural waste in biocatalysis and enzyme technology.