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Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection
Jinlu Tang1, Xiaoxiao He, Yanli Lei
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, Hunan 410082, China. kmwang@hnu.edu.cn huishi_2009@hnu.edu.cn.
Abstract:
A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.
