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A Method to Identify Nucleolus-Associated Chromatin Domains (NADs)
Marie-Christine Carpentier1,2, Ariadna Picart-Picolo1,2, Frédéric Pontvianne3,4
1Laboratoire Génome et Développement des Plantes, CNRS, UMR5096, 66860, Perpignan, France.
Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2017
Summary
Understanding the epigenome requires considering nuclear organization. This study details a protocol to identify Nucleolus Associated chromatin Domains (NADs) in Arabidopsis, applicable to other crops.
Area of Science:
- Plant biology
- Epigenetics
- Nuclear organization
Background:
- The epigenome and its organization are crucial for gene expression.
- Heterochromatin in Arabidopsis localizes to the nuclear and nucleolar periphery.
- Identifying chromatin domains at the nuclear periphery in plants is challenging.
Purpose of the Study:
- To describe a protocol for identifying Nucleolus Associated chromatin Domains (NADs) in Arabidopsis.
- To provide a method for studying the relationship between nuclear organization and gene expression in plants.
Main Methods:
- Isolation of pure nucleoli from Arabidopsis.
- Development of a protocol to identify NADs using nucleolus markers.
Main Results:
- A reliable protocol for identifying NADs in Arabidopsis has been established.
- The protocol allows for the study of chromatin organization relative to the nucleolus.
Conclusions:
- The described protocol enables the identification of NADs in Arabidopsis.
- This method is broadly applicable to other crop species with the transfer of a nucleolus marker.
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