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Updated: Feb 20, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Phase mask-based multimodal superresolution microscopy.

Ryan Beams¹, Jeremiah W Woodcock¹, Jeffrey W Gilman¹

¹Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, MD, 20899.

|October 24, 2017

Summary

This summary is machine-generated.

This study introduces a superresolution microscopy method using phase masks for enhanced imaging. The technique achieves over 120 nm resolution, improving multimodal imaging capabilities.

Keywords:

Microscopy Nonlinear microscopy Superresolution

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Area of Science:

Optics and Photonics
Microscopy
Biophysics

Background:

Superresolution microscopy aims to overcome the diffraction limit for higher resolution imaging.
Existing techniques often require specific fluorescent labels or complex setups.
Multimodal imaging provides complementary information but can be limited by resolution.

Purpose of the Study:

To develop and demonstrate a multimodal superresolution microscopy technique.
To achieve sub-diffraction limit resolution using phase masks and spatial filtering.
To validate the technique across different imaging modalities and samples.

Main Methods:

Utilized a phase-masked excitation beam and spatially filtered detection.
Developed theoretical models for non-paraxial beam focusing with phase masks for linear and two-photon excitation.
Experimentally validated using two-photon luminescence from gold nanoparticles and fluorescence lifetime imaging of polystyrene beads.

Main Results:

Demonstrated a multimodal superresolution microscopy technique with > 120 nm resolution (λ/7).
Acquired simultaneous two-photon fluorescence lifetime, two-photon luminescence, and second harmonic images.
Showcased the technique's versatility with a mixture of fluorescent molecules and gold nanoparticles.

Conclusions:

The developed phase-masked microscopy technique significantly enhances resolution in multimodal imaging.
The approach is adaptable to various scanning-based microscopies by engineering excitation and collection volumes.
This method offers a versatile platform for advanced imaging applications.