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Related Experiment Video

Updated: Feb 20, 2026

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
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Enabling 3D hepatocyte spheroids for microphysiometry.

S Eggert, F A Alexander, J Wiest

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 25, 2017
    PubMed
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    This study introduces a novel spheroid encapsulation method for Organ-on-Chip platforms, enabling continuous monitoring of 3D tissue models for improved toxicology studies.

    Area of Science:

    • Biotechnology
    • Tissue Engineering
    • Microfabrication

    Background:

    • Organs-on-Chips (OoCs) are advanced in vitro platforms for toxicology studies, mimicking in vivo conditions.
    • Continuous monitoring of physiological changes in 3D tissue models is crucial for next-generation OoCs.
    • Current OoCs lack integrated monitoring capabilities for 3D cultures.

    Purpose of the Study:

    • To develop a novel spheroid encapsulation technique for integrating 3D tissue models with monitoring sensors.
    • To enable fluidic communication between 3D models and Intelligent Mobile Lab for In Vitro Diagnostics (IMOLA-IVD) BioChip sensors.
    • To prevent spheroid fusion while allowing for continuous physiological analysis.

    Main Methods:

    • Designed a novel spheroid encapsulation method for microwell-based 3D models.

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  • Developed optimized spheroid culturing protocols for enhanced growth and integrity.
  • Evaluated spheroid integrity on various porous layers for defined encapsulation on BioChip sensors.

    • Main Results:

    • Successfully developed a method for spheroid encapsulation in microwells.
    • Established optimized protocols for spheroid culturing and integrity.
    • Demonstrated the feasibility of integrating 3D models with IMOLA-IVD BioChip sensors.

    Conclusions:

    • The novel spheroid encapsulation addresses the limitations of current in vitro platforms.
    • This technique enables seamless integration of 3D tissue models with monitoring sensors for advanced toxicology.
    • The developed protocols support optimized spheroid growth and integrity for reliable in vitro diagnostics.