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Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma
I P Smirnova1, E V Karimova2, Yu A Shneider2
1T. T. Berezov Department of Biochemistry, Medical Faculty of the Medical Institute, the Peoples' Friendship University of Russia, Moscow, Russia. smir-ip@yandex.ru.
Bulletin of Experimental Biology and Medicine
|October 25, 2017
Summary
Researchers investigated the antibacterial effects of Trichoderma harzianum Rifai F-180 metabolites and its L-lysine-α-oxidase enzyme against Erwinia amylovora. The study confirmed the enzyme
Area of Science:
- Microbiology
- Enzymology
- Biochemistry
Background:
- Trichoderma harzianum Rifai F-180 is known for producing L-lysine-α-oxidase.
- Erwinia amylovora is a highly virulent plant pathogenic bacterium.
Purpose of the Study:
- To evaluate the antibacterial activity of Trichoderma harzianum Rifai F-180 metabolites and its L-lysine-α-oxidase enzyme against Erwinia amylovora.
Main Methods:
- Culturing of Trichoderma harzianum Rifai F-180.
- Preparation of metabolite concentrate and homogenous L-lysine-α-oxidase.
- Assaying enzyme activity (5.4 U/ml for concentrate, 50 U/ml for homogenous enzyme).
- Testing antibacterial effects on Erwinia amylovora.
Main Results:
- Antibacterial activity of L-lysine-α-oxidase against Erwinia amylovora was demonstrated.
- The homogenous enzyme exhibited higher activity (50 U/ml) compared to the metabolite concentrate (5.4 U/ml).
Conclusions:
- L-lysine-α-oxidase derived from Trichoderma harzianum Rifai F-180 possesses significant antibacterial properties.
- The enzyme shows potential for controlling Erwinia amylovora infections.
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