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Specific Cell (Re-)Programming: Approaches and Perspectives

Frauke Hausburg1,2, Julia Jeannine Jung1,2, Robert David3,4

  • 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.

Advances in Biochemical Engineering/Biotechnology
|October 27, 2017
PubMed

Insights

Cellular reprogramming offers new regenerative medicine strategies, overcoming limitations of stem cells for drug development and disease modeling. This review highlights advances in generating functional cells, particularly cardiac pacemaker cells.

Area of Science:

  • Regenerative Medicine
  • Cellular Biology
  • Developmental Biology

Background:

  • Organ dysfunction and limited self-renewal hinder regeneration.
  • Pluripotent stem cells (ESCs, iPSCs) face ethical and safety concerns.
  • Adult stem cells have limited availability for therapeutic applications.

Purpose of the Study:

  • To review recent advances in cellular reprogramming for regenerative medicine.
  • To highlight progress in generating specific somatic cell types.
  • To focus on the generation of cardiac pacemaker cells.

Main Methods:

  • Direct reprogramming protocols using lineage-specific transcription factors.
  • Exogenous expression of coding and noncoding RNAs.
  • Application of chemical compounds for cell generation.

Main Results:

  • Successful direct reprogramming protocols for various somatic cell types.
  • Emerging methods for safe and efficient generation of specified cells.
  • Significant progress in generating cardiomyocyte subtypes, including pacemaker cells.

Conclusions:

  • Cellular reprogramming is crucial for advancing regenerative medicine.
  • Development of functional cell generation protocols supports research and clinical applications.
  • Focus on mature, physiologically relevant cells is key for therapeutic success.
Keywords:
Cardiovascular regenerationCell fate conversionDirect reprogrammingLineage conversionMetabolic disordersNeurodegenerative disordersRegenerative medicine

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