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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

16.6K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Feb 20, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
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Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

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Flow Cytometry: The Glass Is Half Empty.

Howard M Shapiro1

  • 1One World Cytometry, Inc., 283 Highland Ave., West Newton, MA, 02465-2513, USA. hms@shapirolab.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2017
PubMed
Summary
This summary is machine-generated.

This chapter explores the limitations of flow cytometry, highlighting what it cannot achieve. It then introduces alternative, cost-effective, and sustainable simple imaging cytometer methods for broader applications.

Keywords:
CD4 countersHIVImaging cytometersMalaria parasitesTB bacteria

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Flow cytometry is a powerful technique for cell analysis.
  • However, its capabilities are not exhaustive, and certain applications remain challenging.
  • Understanding these limitations is crucial for advancing cell analysis methodologies.

Purpose of the Study:

  • To critically evaluate the limitations of flow cytometry.
  • To introduce alternative imaging cytometry techniques.
  • To promote the use of affordable and sustainable cell analysis tools.

Main Methods:

  • A review of flow cytometry's technical constraints.
  • Exploration of simple imaging cytometer principles.
  • Assessment of cost-effectiveness and sustainability of alternative methods.

Main Results:

  • Flow cytometry has inherent limitations in specific cell analysis scenarios.
  • Simple imaging cytometers offer viable alternatives for certain applications.
  • These alternative methods are often more affordable and sustainable.

Conclusions:

  • Acknowledging flow cytometry's limitations is essential for scientific progress.
  • Simple imaging cytometry presents a promising, accessible alternative.
  • Adoption of sustainable imaging techniques can broaden research accessibility.