Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Current Trends in Nursing I01:28

Current Trends in Nursing I

5.5K
Current trends in nursing include:
5.5K
Current Trends in Nursing II01:30

Current Trends in Nursing II

3.6K
Trends in nursing are multifactorial and associated with changes in society, within the nursing profession, and in other professions. Notably, telehealth and remote nursing contribute to successful healthcare delivery for numerous patients and help reduce stress for nurses due to nursing shortages. Nurses can reach patients, monitor their conditions, and interact with them using computers, audio, visual accessories, and telephones—for example, remote patient monitoring systems. Likewise,...
3.6K
Flow Cytometry01:23

Flow Cytometry

16.5K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.5K
Issues And Trends In Healthcare Delivery System01:29

Issues And Trends In Healthcare Delivery System

6.3K
The issues and trends in healthcare delivery are constantly changing. The COVID-19 pandemic is one recent issue that wreaked havoc on healthcare systems, causing a shortage of healthcare workers, high demand for medicines and supplies, and increased medical expenditure due to a lack of insurance. Other issues include rising healthcare costs and care fragmentation.
Cost Containment
Payment for healthcare services has historically promoted adoption of costly and often unnecessary or inefficient...
6.3K
Electrical Current01:10

Electrical Current

7.2K
Electrical current is defined as the rate at which charge flows. When there is a large current present, such as that used to run a refrigerator, a large amount of charge moves through the wire in a small amount of time. If the current is small, such as that used to operate a handheld calculator, a small amount of charge moves through the circuit over a long period of time. The SI unit for current is the ampere (A), named for the French physicist André-Marie Ampère (1775–1836).
7.2K
Trends in Lattice Energy: Ion Size and Charge02:54

Trends in Lattice Energy: Ion Size and Charge

26.8K
An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. For the ionic solid sodium chloride, the lattice energy is the enthalpy change of the process:
26.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intercellular nanotube-mediated mitochondrial transfer enhances T cell metabolic fitness and antitumor efficacy.

Cell·2024
Same author

LIM-domain-only 4 (LMO4) enhances CD8<sup>+</sup> T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling.

Signal transduction and targeted therapy·2024
Same author

Flow Cytometry: The Glass Is Half Full.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Basic Principles of Flow Cytometer Operation: The Make your Own Flow Cytometer.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Laser Sources for Traditional and Spectral Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Multiparametric Analysis of Apoptosis by Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2024
Same journal

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

Current protocols in cytometry·2020
Same journal

Live Imaging of the Lung.

Current protocols in cytometry·2020
Same journal

Small Particle Fluorescence and Light Scatter Calibration Using FCM<sub>PASS</sub> Software.

Current protocols in cytometry·2020
Same journal

Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.

Current protocols in cytometry·2020
Same journal

Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood.

Current protocols in cytometry·2020
Same journal

Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens.

Current protocols in cytometry·2020
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

18.3K

Lasers for Flow Cytometry: Current and Future Trends.

Howard M Shapiro1, William G Telford2

  • 1The Center for Microbial Cytometry, West Newton, Massachusetts.

Current Protocols in Cytometry
|January 19, 2018
PubMed
Summary
This summary is machine-generated.

This review explores laser light sources for flow cytometry, detailing suitable laser types and future tunable laser advancements for multilaser systems.

Keywords:
Gaussian beamsflow cytometryillumination opticslasers

More Related Videos

Global and Current Research Trends of Single-Cell Sequencing in Cancer: A Bibliometric and Visualization Study
07:50

Global and Current Research Trends of Single-Cell Sequencing in Cancer: A Bibliometric and Visualization Study

Published on: April 18, 2025

1.0K
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
09:11

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

2.8K

Related Experiment Videos

Last Updated: Feb 15, 2026

Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

18.3K
Global and Current Research Trends of Single-Cell Sequencing in Cancer: A Bibliometric and Visualization Study
07:50

Global and Current Research Trends of Single-Cell Sequencing in Cancer: A Bibliometric and Visualization Study

Published on: April 18, 2025

1.0K
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
09:11

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

2.8K

Area of Science:

  • Biophotonics and Instrumentation
  • Optical Engineering

Background:

  • Lasers are essential light sources in flow cytometry.
  • Most flow cytometers utilize multiple lasers for comprehensive analysis.

Purpose of the Study:

  • To review laser types suitable for flow cytometry.
  • To discuss future trends in tunable laser development.
  • To provide practical guidance for constructing multilaser systems.

Main Methods:

  • Review of available laser technologies.
  • Analysis of laser characteristics for flow cytometry applications.
  • Discussion of system integration strategies.

Main Results:

  • Identified key laser properties influencing flow cytometer performance.
  • Highlighted the potential of tunable lasers for enhanced capabilities.
  • Offered practical advice for building complex multilaser setups.

Conclusions:

  • Selection of appropriate lasers is critical for effective flow cytometry.
  • Advancements in tunable lasers promise expanded applications.
  • Guidance for multilaser system construction aids researchers.