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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Feb 20, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Flow Cytometry: The Glass Is Half Full.

Howard M Shapiro¹

¹One World Cytometry, Inc., 283 Highland Ave., West Newton, MA, 02465, USA. hms@shapirolab.com.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2017

Summary

This summary is machine-generated.

This chapter explores the capabilities of flow cytometry, a powerful cell analysis technique. It highlights the vast potential and applications of this technology in scientific research.

Keywords:

Blood cells Cells Coulter volume Dyes Electrostatic sorting Flow cytometry Fluorescence Hemacytometry Micrographia Microscopy Poisson statistics

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Last Updated: Feb 20, 2026

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Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Provides a historical overview of cytometry.
Introduces the fundamental principles and instrumentation of flow cytometry.
Establishes the context for understanding the capabilities of flow cytometry.

Discussion:

Explores the diverse applications of flow cytometry across various scientific disciplines.
Highlights the versatility and adaptability of flow cytometry techniques.
Emphasizes the 'glass half full' perspective, focusing on the extensive possibilities offered by flow cytometry.

Key Insights:

Flow cytometry is a versatile technology with a rich history.
The chapter offers a comprehensive overview of flow cytometry's current capabilities.
Numerous applications exist, demonstrating the technology's significant potential.

Outlook:

Discusses emerging trends and future directions in flow cytometry.
Suggests areas for further research and development in the field.
Encourages exploration of novel applications and advancements in flow cytometry technology.