[Multiomics study of HepG2 cell line proteome].

Area of Science:

• Proteomics
• Genomics
• Molecular Biology

Background:

• Current proteomic studies primarily focus on abundant proteoforms from canonical sequences.
• Advancements in transcriptomic and proteomic technologies enable the identification of non-canonical proteoforms.
• Aberrant proteoforms arise from alternative splicing, single nucleotide polymorphism, and post-translational modifications.

Purpose of the Study:

• To estimate the heterogeneity of the HepG2 proteome.
• To explore the potential of a multi-omics approach for comprehensive proteome analysis.
• To identify aberrant proteoforms in HepG2 cells.

Main Methods:

• Utilized a multi-omics strategy combining transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods.
• Analyzed HepG2 cell samples to capture a broad spectrum of proteoforms.
• Employed state-of-the-art analytical technologies for data acquisition and analysis.

Main Results:

• The study provides insights into the complexity and heterogeneity of the HepG2 proteome.
• Demonstrated the feasibility of identifying non-canonical proteoforms using the integrated approach.
• Characterized a range of proteoforms, including those resulting from alternative splicing, SNPs, and PTMs.

Conclusions:

• A multi-omics approach integrating RNAseq and 2DE-MS/MS is a powerful strategy for exploring proteome heterogeneity.
• This methodology facilitates the identification of aberrant proteoforms, expanding our understanding of cellular proteomes.
• The findings contribute to a more comprehensive view of the HepG2 proteome, moving beyond canonical sequences.