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Updated: Feb 19, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Published on: June 25, 2010
Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas
Róbert Kubinec1, Peter Kotora2, Viktória Ferenczy1,3
1Institute of Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia.
Abstract:
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL-1 for mannitol to 4.7 mg/L for glucose.
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