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Single fiber reflectance spectroscopy calibration.

Xu U Zhang1, Anouk L Post1, Dirk J Faber1

  • 1Academic Medical Center, University of Amsterdam, Department of Biomedical Engineering and Physics,, The Netherlands.

Journal of Biomedical Optics
|November 1, 2017
PubMed
Summary
This summary is machine-generated.

Accurate optical property determination using single fiber reflectance spectroscopy (SFR) requires reliable calibration. The Fresnel reflection method at the fiber tip proved most reproducible and accurate for absolute reflectance calibration of SFR.

Keywords:
calibrationmeasured absolute reflectancesingle-fiber reflectance spectroscopy

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Optical Engineering

Background:

  • Accurate measurement of sample optical properties is crucial for applications using single fiber reflectance spectroscopy (SFR).
  • Absolute calibration of reflectance is a prerequisite for reliable SFR measurements.
  • Existing calibration methods have limitations in reproducibility and accuracy.

Purpose of the Study:

  • To investigate and compare different methods for absolute reflectance calibration of single fiber reflectance spectroscopy (SFR).
  • To identify the most reliable and reproducible calibration method for SFR.

Main Methods:

  • Evaluated two novel SFR calibration methods: using a calibrated mirror and utilizing the Fresnel reflection at the fiber tip.
  • Compared these methods against established techniques using Intralipid-20% with Monte Carlo simulations and Spectralon as references.

Main Results:

  • The Fresnel reflection method demonstrated superior reproducibility compared to other investigated methods.
  • This method yielded the most reliable results for absolute reflectance calibration in SFR.
  • Calibrated mirror and Intralipid/Monte Carlo methods showed lower reproducibility.

Conclusions:

  • The Fresnel reflection at the fiber tip is recommended as the preferred method for absolute reflectance calibration of SFR.
  • This method offers enhanced reliability and reproducibility for optical property determination.
  • Advancements in SFR calibration are critical for accurate biomedical optics applications.