INFLUENCE OF TESTOSTERONE ON MORPHOLOGICAL CHANGES IN PANCREATIC ISLETS AND LIVER TISSUE OF RATS WITH ALLOXAN-INDUCED...

The role of exogenous testosterone (methyltrienolone) in the repair of damaged pancreatic islet and liver cells during alloxan diabetes was studied. Modeling of diabetes was performed through single intraperitoneal injection of Alloxan, with the dose 2 mg per 10 g of body weight. Pancreatic and liver tissues were investigated microscopically using 3-4 µm slices embedded in paraffin and stained with hematoxylin and eosin. Software Adobe Photoshop was used to measure the areas of pancreatic islets as well as areas of necrotic foci in these islets of animals with Alloxan-induced experimental diabetes. The degree of liver damage was assessed by a 5-score "visual scale". The animals with diabetes revealed degenerative changes of pancreatic islets and liver tissue, the intensity of which correlates with the time-period passed since diabetic modeling. These changes correspond to the metabolic abnormalities described for various experimental models or clinical cases of diabetes. The study revealed that treatment of Alloxan-induced diabetes with testosterone (methyltrienolone) restores not only the biochemical disorders (as previously described) but also supports to reverse the pathological changes in pancreatic and liver tissues.